Optical and magnetic resonance imaging using upconversion nanoparticles

Summary

The project will involve the synthesis of advanced upconversion nanoparticles and their characterisation aimed at assessing their suitability as dual-mode (optical and MRI) imaging materials for cancer tissues. Upconversion nanoparticles are inorganic nanoparticles that are capable of emitting visible light under near-infrared laser excitation allowing us to image deeper into the tissues with less interference compared to other light-based imaging techniques.

The synthesis of upconversion nanoparticle (UCNP) based systems and their surface functionalisation with selected Affimers/antibodies for dual-mode imaging applicable to cancer tissues is the overall objective of this project. In this project we propose to develop different types of UCNPs doped with different rare earth ion sensitizer and activator combinations (Yb3+, Er3+, Tm3+ and Ho3+) will be developed. The factors such as type of precursors, surfactants other preparative conditions are varied to obtain particles having different shapes, phase and high photoluminescence intensity. Surface modification with various polymers and other suitable methods (such as coating with silica) followed by conjugation of Affimers will allow targeted imaging. Development of particle systems which suits dual modal imaging (Optical/magnetic) applications seek importance. The cytotoxicity analysis of particles of interest will be carried out using MTT assay, NRU assay or comet assay, which could be assessed before preclinical trials.

Advanced structural characterisation of the UCNP systems using electron microscopy, X-ray diffraction, and laser spectroscopy will be undertaken prior to their use in imaging experiments. The imaging experiments will be carried out in collaboration with Leeds Teaching Hospitals and the School of Medicine and Health. In the dual model imaging, the student has to develop the functionally modified particle with optically and magnetically active centres in order to acquire optical and MRI image.

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