Using cryo-electron microscopy to study large protein complexes that mediate the female post-mating response

Summary

In many animals, male seminal fluid alters female physiology and post-mating behaviour to ensure paternity of the offspring. However, how seminal fluid components elicit these post-mating responses is poorly understood. To investigate this question, in this PhD project, you will use cryo-electron microscopy to solve the molecular structures of large seminal fluid protein complexes, and characterise their function in Drosophila reproduction using biochemical and genetic approaches.

You will work jointly in the groups of Dr Joe Cockburn and Prof. Elwyn Isaac at the Astbury Centre for Structural and Molecular Biology, a vibrant interdisciplinary research centre at the University of Leeds with world-class research facilities for structural biology. No prior experience in electron microscopy is required, but you must have a passion for structural biology, a desire to understand biological function at the molecular level and an interest in how this influences animal behaviour.

Applicants to research degree programmes should normally have at least a first class or an upper second class British Bachelors Honours degree (or equivalent) in an appropriate discipline. The criteria for entry for some research degrees may be higher, for example, several faculties, also require a Masters degree. Applicants are advised to check with the relevant School prior to making an application. Applicants who are uncertain about the requirements for a particular research degree are advised to contact the School or Graduate School prior to making an application.

The minimum English language entry requirement for research postgraduate research study is an IELTS of 6.0 overall with at least 5.5 in each component (reading, writing, listening and speaking) or equivalent. The test must be dated within two years of the start date of the course in order to be valid. Some schools and faculties have a higher requirement.